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The research team used a typical amphiphilic molecule as a research object and used terahertz spectroscopy to analyze the change of molecular hydration layer during the change of surfactant molecules from monomer to micelle: when the critical micelle concentration (CMC) was lower than The solution terahertz absorption coefficient is negatively correlated with the concentration; higher than the CMC, the solution terahertz absorption coefficient is positively correlated with the concentration, and accordingly, a terahertz spectroscopic technique is used to detect the surfactant critical micelle concentration (CMC) without a label. Methods.
In the acid solution environment, the increase of H3O makes the hydration network in the liquid environment enhance and increase the absorption coefficient of the solution. The decrease of the ionic surfactant CMC and the increase of the ionic surfactant CMC indicate different amphiphilic molecules and water molecules There are differences in interactions.
The energy level of biomolecules interacting with water is in the terahertz waveband. In this frequency range, it exhibits strong absorption and resonance. The information contained in the terahertz spectra that cannot be detected in other electromagnetic wave bands is an important part of understanding the basic physicochemical processes of life activities. Based on the basic data, the use of terahertz spectra to obtain biomolecular information has become a hot topic in the academic community.
As natural amphiphilic substances, lipids are the main component of the cell membrane system and an important part of all cells. Continue to use the terahertz spectroscopy technology to study natural amphipathic compounds will provide a theoretical basis for in-depth understanding of the dynamic changes of cell membrane structure. .
(Original title: Chongqing Research Institute made progress in terahertz spectral studies of amphiphilic molecules in aqueous solution)
ã€Chinese Instrument Network Instrument Development】 Recently, the research team of the Terahertz Technology Research Center of the Chongqing Institute of Green Intelligent Technology, Chinese Academy of Sciences, used terahertz spectroscopy to study the interaction between amphiphilic compounds and water in liquid environments. Critical Micelle Concentrations of Surfactants by Terahertz Time-Domain Spectroscopy was published in IEEE Transactions on Terahertz Science and Technology (DOI: 10.1109/TTHZ.2016.2575450).